Good centrifuge practice
To ensure peak performance of your MSE centrifuge, you should abide by the following steps:
- – Always run with a full set of buckets.
- – Distribute load evenly around the rotor.
- – Balance opposing buckets to within 5 grams.
- – Regularly clean and decontaminate your rotor, buckets and adapters, particularly if there is a spillage.
- – Use a brush with soft bristles for cleaning. Avoid using a brush with wire bristles.
- – Dry buckets and rotors after cleaning to prevent corrosion. Dry upside down so excess water can drain away.
- – Spray buckets with an anti-corrosion spray once dried.
- – Ensure rotor, buckets and windshield lids are securely fitted.
- – Ensure the nut is securely fitted on the rotor.
- – Never run a windshield rotor without the lid.
- – Remove any broken tubes or debris from bowl.
- – For refrigerated units, you should leave lid open after use to allow moisture to evaporate and prevent corrosion.
- – Rotors and buckets should be replaced after 10 years or 30,000 cycles (whichever the greater).
Most importantly, centrifuges need to be regularly maintained and serviced. As with a car, a single annual service can extend the life of your centrifuge considerably as well as increase reliability and safety. Centrifuges that are not properly maintained can be potentially dangerous. Furthermore, if a user is inappropriately trained or inexperienced, then this too can be hazardous.
You can contact MSE directly if you have any questions relating to the operation of your centrifuge. Alternatively, your nearest service engineer will be able to visit your site and give basic training, as well as repair and maintain your equipment.
Calculation of G force or RCF (Relative Centrifugal Force).
RCF is dependent on the speed of the rotor and the distance from the centre of the motor drive shaft to the farthest point of the sample tube from the centre of the rotor (not the edge of the bucket or rotor). When you buy your MSE centrifuge, you will know the maximum speed and RCF of the rotor and its radius. However, for any speed other than maximum, you will need to calculate the RCF.
If you do not know the radius of the rotor, you will have to measure this. Sometimes this may entail a little bit of guesswork in the case of an angled rotor. The radius is measured from the centre of the rotor to the point that would be the bottom of the sample tube. Make sure you take into account any inserts and tube cushions that may be used.
The formulas for calculating RCF and rpm are as follows: